Fig. 7
From: Histone lysine dimethyl-demethylase KDM3A controls pathological cardiac hypertrophy and fibrosis
JIB-04 protects mice against I/R injury. WT mice were subjected to I/R surgery and given vehicle (Veh) or JIB-04 as shown in Fig. 6a. Echocardiographs were performed weekly for 4 weeks to measure the fractional shortening (a). b Percent fibrotic area and c HW/BW ratio of mice treated with Veh or JIB-04 at 4 weeks post-I/R surgery. d Immunofluorescences of H3K9me2, H3K9me3, H3K4me3, and H3K27me3 of hearts treated with Veh or JIB-04 at week 4 post-I/R surgery. H3K9me2/3 in both cardiomyocyte (arrow head) and non-cardiomyocytes (arrow) were affected by JIB-04 treatment. Scale bar, 20 μm. e Quantification of percent of cells in d stained with methylated histones as indicated. Staining of methylated histones was normalized against Dapi stained cells. *p < 0.05, n = 5 ± SEM (ANOVA)
