Fig. 5

Immunogenicity of loop-swapped HPV33/58/52 chimeric VLPs at three dosages and other triple-type chimeric VLPs. a Groups of mice (n = 5) were immunized with 0.1, 1 or 10 μg per dose of selected chimeric or WT VLPs at weeks 0, 2 and 4. Immune sera were collected at week 8 and analyzed by neutralization assays. b Neutralization titers of immune sera (at week 8) in BALB/c mice (n = 5) immunized with 5 μg per dose of chimeric and WT L1 proteins of Groups 2, -3, -5, and -6 (also see Fig. 1a) in aluminum adjuvant at weeks 0, 2 and 4. All the data were analyzed by one-way ANOVA, **P < 0.01; ***P < 0.001; ****P < 0.0001. The error bars are standard deviation and symbols represent individual mice. The dotted line indicates the limit of detection for the assay. Non-neutralizing sera were assigned a value of one-half of the limit of detection for visualization