Fig. 5

Propargite induces β-cell toxicity in vivo. Fasting mouse insulin levels, and body weights in control (corn oil) or 12 mg/kg propargite-treated mice (n = 10 mice). p values calculated by one-way repeated measures ANOVA. b, c Representative images (b) and DNA damage rate (c) of mouse β-cells in mouse pancreas (n = 7 images for corn oil treated mice and n = 10 images for Propargite-treated mice). DNA damage rate of mouse β-cells was calculated as the percentage of γ-H2A.X ⁺/INS⁺ cells in INS⁺ cells. Scale bars, 100 μm. d Scheme of the in vivo imaging analysis. Day 0 is the day the mice are first administrated with corn oil (vehicle) or propargite. e Fold-change of luciferase signals from EndoC-βH1-Luc cells transplanted mice administrated with corn oil (vehicle) or propargite during the 5-day course (n = 8 mice). p values calculated by one-way repeated measures ANOVA. f, g Representative images of TUNEL and INS staining (f) and Quantification of %TUNEL⁺ cells (g) in EndoC-βH1-Luc cells carrying sgGSTT1 or sgGFP on day 3 (n = 3 images for each condition). p values calculated by two-way repeated measures ANOVA with a Bonferroni test for multiple comparisons between wt and mutant cells. Related to Supplementary Fig. 6