Fig. 4 | Nature Communications

Fig. 4

From: Multi-color live-cell super-resolution volume imaging with multi-angle interference microscopy

Fig. 4

Multi-angle interference imaging of mitochondria. a Lateral super-resolution image and b 3D volume super-resolution image of mitochondria. The bottom-right of a shows the corresponding TIRF image. The bottom and right images of b show the corresponding vertical cross-section along the respective orange and purple dotted lines in b produced using ImageJ3D. c Consecutive xy sections of the green boxed region in b from a 0 to 180-nm depth, showing the hollow shape and morphology change of individual mitochondria. d Three-dimensional (3D) volume visualization of the mitochondria using ImageJ3D. The size of the reconstructed 3D region is 32 × 32 × 0.6 μm3. Scale bars, 5 μm (a, b), and 10 μm (bottom-right of a)

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