Fig. 5

Multi-color multi-angle interference imaging of microtubules and mitochondria. a Lateral super-resolution image of microtubules stained with Alexa Fluor 488 (λ = 488 nm) and b outer mitochondrial membrane 20 (TOM20) stained with Alexa Fluor 568 (λ = 561 nm) in the same U373 cells. The bottom-right of a and b shows the corresponding TIRF images. c Dual-color merging lateral super-resolution co-localization image of microtubules (green) and mitochondria (magenta). d Three-dimensional (3D) volume super-resolution image of the same microtubules and e mitochondria. The h of the microtubules and mitochondria is 190 and 142 nm, respectively. f Merging super-resolution volume co-localization image of microtubules and mitochondria. Scale bars, 4 μm (a–f), and 8 μm (bottom-right of a and b)