Fig. 6

Live-cell time-lapse multi-angle interference imaging of mitochondria and microtubules. a Lateral super-resolution image of mitochondria in live U2OS cells labeled with Atto 647N (λ = 640 nm). The bottom-right shows the corresponding TIRF image. b Time-lapse 3D volume super-resolution image sequence of the ROI marked with a white box in a, showing the changes of the lateral and axial positions and the fission (purple triangle) and fusion (orange triangle) of mitochondria. The h varies between 296 nm and 438 nm during the interval of 20 s to 30 s. c Lateral super-resolution image of microtubules in live U2OS cells labeled with SiR-tubulin (λ = 640 nm). The bottom-right shows the corresponding TIRF image. d A time-lapse 3D volume super-resolution image sequence of the ROI marked with a white box in c, which shows the changes of lateral and axial positions of microtubules. The h changes from 138 nm to 32 nm during the interval of 0 s to 45 s. Scale bars, 10 μm (a, d), and 5 μm (bottom-right of a and b)