Fig. 3
From: Structural insights into the function of type VI secretion system TssA subunits
Effect of TssA1B inactivation on T6SS secretion activity. Cell associated protein (CA) and spent supernatants (SN) from cultures of B. cenocepacia H111 (WT) and the isogenic tssA1B mutant (tssA::Tp), each with or without pBBR1MCS (VC) or pBBR1MCS-tssA1B (ptssA), were fractionated by SDS-PAGE, blotted onto PVDF membrane and probed with a Hcp pAb or a mAb specific for the RNAP β subunit (lysis control). Detection of bound antibodies was with HRP-conjugated secondary antibodies. The tssM mutant (ΔM) was included as a control as TssM has been shown to be an essential component of the B. cenocepacia T6SS31. Uncropped images of the blots are shown in Supplementary Fig. 5
