Fig. 4

T_reg-cell-specific overexpression of Id2 results in systemic autoimmunity in older mice. a A representative picture of 12–16 week-old Foxp3^YFP−Cre and Id2^EmGFPFoxp3^YFP−Cre mice (left) and spleen (SP) and peripheral lymph nodes (pLN) derived from them (right). b Representative hematoxylin and eosin (H&E)-stained skin, lung, liver sections from 12–16 weeks old Foxp3^YFP−Cre and Id2^EmGFPFoxp3^YFP−Cre mice (scale bar, 100 μm). c Representative FACS plots and percentages of CD4⁺ and CD8⁺ T cells from SP and pLN of 12–16 weeks old Foxp3^YFP−Cre and Id2^EmGFPFoxp3^YFP−Cre mice. d, e Representative FACS plots and percentages of CD62L^hiCD44^lo (naive) and CD62L^loCD44^hi (effector/memory) cells among CD4⁺ and CD8⁺ T cells in SP and pLN 12–16 week-old mice. f Representative FACS plots and percentages of Foxp3⁺ T_reg cells in thymus, SP and pLN from 12–16 week-old mice. NS,  not significant, *P < 0.05, **P < 0.005 (Student’s t-test). All data are representative three independent experiments (error bars, s.d.)