Fig. 6
From: Inflammation-induced Id2 promotes plasticity in regulatory T cells
Id2 expression inhibits enrichment and transcriptional activity of E2A on Foxp3 promoter. a RT-qPCR analysis of Foxp3, E2A and Id2 mRNA in in vitro generated TH0, TH17, ex-Foxp3 TH17, and iTreg cells. b ChIP-qPCR analysis for E2A occupancy at three putative E-box sites on Foxp3 promoter regions (−1593 to −1584, −1295 to −1286, and −837 to −829) and negative control (−411 to −244; No E-box site) in iTreg and ex-Foxp3 TH17 cells. Enrichments are calculated relative to the input chromatin for corresponding sites. c Jurkat cells were transfected with either Foxp3 promoter reporter construct alone or along with expression plasmids encoding E2A and Id2 as indicated, followed by luciferase assay. d Effect of mutagenesis on Foxp3 promoter reporter activity. Jurkat cells were transiently co-transfected with the indicated plasmids for luciferase assay as described. N = Normal E box, X = Mutated E box. NS, not significant, *P < 0.05, **P < 0.005, ***P < 0.001 (Student’s t-test). All data are representative of two or three independent experiments (error bars, s.d.)
