Fig. 2
From: Serotonin signals through a gut-liver axis to regulate hepatic steatosis
Gut-derived 5-HT does not affect systemic energy metabolism. a–n The 12-week-old WT and Tph1 GKO mice were fed SCD or HFD for 8 weeks. a Body weight trends; n = 6–10 per group. b Intraperitoneal glucose tolerance test (IPGTT) after 16 h fasting; n = 5 per group. c Intraperitoneal insulin tolerance test (IPITT) of HFD-fed WT and Tph1 GKO mice after 4 h fasting; n = 5 per group. d Plasma total cholesterol, free fatty acid, triglyceride, and high-density lipoprotein (HDL) cholesterol levels; n = 4–5 per group. e Percent fat body mass and lean body mass of HFD-fed WT and Tph1 GKO mice; n = 3 per group. f, h, j Adipose tissue to body weight ratio of BAT (f), iWAT (h), and eWAT (j); n = 6–10 per group. g, i, k Representative histology by H&E staining of sections from BAT (g), iWAT (i), and eWAT (k). Scale bars, 100 μm. l, m Relative mRNA expression of Ucp1 assessed by qRT-PCR in BAT (l) and iWAT (m) of HFD-fed WT or Tph1 GKO mice; n = 3 per group. n Metabolic parameters of HFD-fed WT or Tph1 GKO mice; n = 4 per group. Data are expressed as the means ± SEM. *P < 0.5, ***P < 0.001, Student’s t-test (c, e, l, m) or one-way ANOVA with post hoc Tukey’s test (a, b, d, f, h, j, n). VO2: oxygen consumption, VCO2: carbon dioxide production, RER: respiratory exchange ratio, XTOT: horizontal motor activity
