Fig. 4
From: Serotonin signals through a gut-liver axis to regulate hepatic steatosis
HTR2A mediates the lipogenic action of GDS in the liver. a Relative mRNA expression of indicated HTRs as assessed by qRT-PCR in liver of SCD- and HFD-fed C57BL/6J mice; n = 4 per group. b–j The 12-week-old WT and Htr2a LKO mice were fed SCD or HFD for 8 weeks. Representative gross liver image (b) and liver weight (c) of HFD-fed WT and Htr2a LKO mice; n = 6–10 per group. Scale bar, 1 cm (b). d Representative liver histology by H&E staining from HFD-fed WT and Htr2a LKO mice. Scale bars, 100 μm. e NAS of HFD-fed WT and Htr2a LKO mice; n = 6–10 per group. f Hepatic triglyceride levels; n = 6–10 per group. g–j Relative mRNA expression of genes involved in lipogenesis (g), FA uptake (h), FA oxidation (i), and VLDL secretion (j) as assessed by qRT-PCR in liver; n = 5 per group. k–m The 8-week-old WT and Htr2a LKO mice were fed MCD diet for 6 weeks. k Representative liver histology by H&E staining. Scale bars, 100 μm. l NAS; n = 6–8 per group. m Hepatic triglyceride levels; n = 6–8 per group. Data are expressed as the means ± SEM. *P < 0.5, **P < 0.01, ***P < 0.001, Student’s t-test (a, c, e, l, m) or one-way ANOVA with post hoc Tukey’s test (f–j)
