Fig. 6
Validation of the hit compounds using behavioral test in PTZ-seizure animal model. a Schematic of the setup for the behavioral test. b The experimental protocol. c The seizure count over 15 min in larvae exposed to 5 mM PTZ after 4-h preincubation with NNC-711 at different concentrations (10 ~ 500 μM). A dose-dependent reduction in seizures is noted. Error bars indicate standard error of the mean (s.e.m.), n = 12, **** indicates p < 0.0001 by one-way ANOVA tests. d Representative results for the movement behavioral responses with NNC-711 preincubation at different concentrations (10 ~ 500 μM). e The statistical analysis of the locomotive behavior of the larvae in response to the preincubation with NNC-711 at different concentrations (10 ~ 500 μM). Each larva’s movement was monitored for 15 min after a 2 min light stimulation. Error bars indicate s.e.m., n = 12. Larvae treated with DMSO were used as a control, “n.s.” stands for no significant difference with p > 0.05 by ANOVA test. f Traces of calcium fluctuation from five larval brains and the associated T-score BAM showing the seizure activity induced by 5 mM PTZ treatment. g Traces of calcium fluctuation from five larval brain and the associated T-score BAM showing the reduction of seizure activity in PTZ-larvae by a 4 h treatment of NNC-711 at 10 µM
