Fig. 6

LMP2A is involved in VM formation and AKT/HIF-1α activation. a RT-PCR analysis of EBV latent genes in CNE2 and HNE1 cells carrying recombinant EBV (Akata strain) and the native EBV-infected cell line C666-1. GAPDH was used as the internal control. b CNE2 and HNE1 cells were transfected with empty vector or construct encoding LMP2A. Activation of AKT/mTOR/HIF-1α signaling pathway was determined by immunoblotting. c Representative images (top) and quantification (bottom) of tube formation on Matrigel of control and LMP2A-overexpressing NPC cells. Scale bars = 100 μm. Tube numbers were counted under a microscope with 200× magnification in randomly chosen fields. Mean ± SD, n = 5, two-tailed Mann–Whitney test. d CNE2-EBV and TW03-EBV cells were transfected with control or LMP2A-specific siRNAs. The knockdown efficiency was determined by RT-PCR, and HIF-1α level and AKT/mTOR/S6K signaling activity were determined by immunoblotting. e Representative images (left) and quantification (right) of tube formation of NPC-EBV cells transfected with control or LMP2A-specific siRNA. Scale bars = 100 μm. Mean ± SD, n = 5, two-tailed Mann–Whitney test. **p < 0.01 and ***p < 0.001