Fig. 6
Syntrophic coculture of S. multivorans and Methanococcus voltae. a Scheme of syntrophic interactions and exchange of metabolites and b lactate concentration in S. multivorans pure culture and coculture of S. multivorans and M. voltae. c Electron microscopic images of aggregates, Magnifications, ×150 (whole aggregate, upper row left, scale bar 200 µm), ×5000 (upper right, scale bar 3 µm), ×10,000 (lower images, scale bar 2 µm). Sections of the lower images were obtained from different areas of the aggregate. White arrows indicate EPS-like structures. Cultivation experiments included three biological replicates in which similar aggregates were formed. S.m. S. multivorans. Pure S. multivorans and the coculture were cultivated in a medium originally optimized for M. voltae modified as described in the Methods section
