Fig. 5
From: PTEN expression by an oncolytic herpesvirus directs T-cell mediated tumor clearance
Safety of HSV-P10. Viral spread of HSVQ (blue line) and HSV-P10 (red line) in cultures of HUVEC (a) and U251T3 tumor cells (b) was assessed using the Cytation 5 live imaging system, where GFP expression was monitored over time. GFP object count was quantified and graphed as an average of 4 wells per treatment group ± SEM. c Immunofluorescent staining of differentiated neurons showing MAP2 and Tuj1 staining. Scale bar = 50 µm. d Infection (GFP) of neurons and MDA468 cells followed by GFP visualization. Data shown are histograms of the indicated cells infected with HSVQ or HSV-P10. e Cytolytic activity (PI/Annexin-V) in neurons was assessed by flow cytometry 24 hpi. Dot blots of annexin and PI staining of infected (GFP positive) neurons and MDA-468 cells
