Fig. 7
From: PTEN expression by an oncolytic herpesvirus directs T-cell mediated tumor clearance
HSV-P10 reduces cell-surface PD-L1 expression. a Histograms of PD-L1 expression as measured by flow cytometry 16 hpi in a panel of human and mouse cancer cell lines. Gates were placed on live cells, and then for GFP positive cells. Relative fluoresence intensity was calculated based upon PD-L1 stain. Red histogram: isotype control, blue histogram: no virus, orange histogram: HSVQ, green histogram: HSV-P10. b Quantification of relative median fluorescence intensity (RFI) of the indicated cells treated with no virus, HSVQ or HSV-P10 and stained with PDL-1 antibody where statistical significance was assessed by one-way ANOVA (n = 3/group, *p < 0.05, ***p < 0.001, ****p < 0.0001). c Changes in PD-L1 expression ( ± s.d.) on uninfected (GFP negative) DB7 cells following low-MOI infection with either HSVQ or HSV-P10 where statistical significance was assessed by one-way ANOVA (n = 3, *p < 0.05, ****p < 0.0001). Gates were placed on live cells, and then for GFP negative cells. Relative fluoresence intensity was calculated based upon PD-L1 stain. d Changes in PD-L1 expression measured by flow cytometry 12 hpi in infected DB7 cells treated with HSVQ ± PI3Ki or HSV-P10 (LY294002). Gating schematics are outlined in supplementary figure 5
