Fig. 3
FoxO1 bound to HDAC3 and NCoR1 to form a corepressor complex. a Arg1 promoter activity after IL-4 stimulation with or without CA-FoxO1 transfection (n = 4–5). b Mutant A, B, and A plus B Arg1 promoter activities after IL-4 stimulation with or without CA-FoxO1 transfection (n = 5–6). c The binding of DNA nucleotides of the B area of the Arg1 promoter and proteins, and the blocking of the DNA-protein complex formation in a dose-dependent manner by FoxO1, HDAC3 and NCoR1 antibody were determined by EMSA in RAW264.7 cells. d Chip-qPCR using FoxO1, HDAC3 and NCoR1 antibody in the BMDM of the C57BL/6 mice before and after IL-4 stimulation (n = 5–10). e Chip-qPCR using FoxO1, HDAC3 and NCoR1 antibody in the BMDM of the MIrs2KO mice before and after IL-4 stimulation (n = 3–16). f Co-immunoprecipitation (Co-IP) with FoxO1, HDAC3 and NCoR1 antibody without IL-4 stimulation in the Raw 264.7 cells and BMDM. g Expression levels of Arg1 in the BMDM of the C57BL/6 mice after IL-4 stimulation with siHDAC3 or siNCoR1 transfection (n = 3–8). The data are mean ± SEM. followed by one-way ANOVA with a post hoc test. *P < 0.05; **P < 0.01; ***P < 0.001
