Fig. 3

In vitro study of miR-141. a miR-141 transfecting cultured primary human NP cells as confirmed by Cy3. Scale bar = 100 μm. b Forty-eight hours after transfection of miR-141 mimics or inhibitor and their negative control, the cells were used for the following experiments. Transfection efficiency of miR-141 was analyzed using qRT-PCR. n = 3 replicates per group, ***p < 0.001 by one-way ANOVA test followed by Tukey’s post hoc. c, d Cell proliferation was analyzed in miR-141 mimics or inhibitor transfected cultured primary human NP cells using CCK8 and EdU assays. n = 3 replicates per group, ***p < 0.001 by one-way ANOVA test followed by Tukey’s post hoc. Scale bar = 100 μm. e Analysis of NP cells apoptosis was assayed by FCM. n = 3 replicates per group. f The expression levels of Col II, aggrecan, MMP13, and ADAMT5 were detected by western blot. Quantitative analysis was shown on the right, and three independent repeats were performed in each experiment. ***p < 0.001 by one-way ANOVA test followed by Tukey’s post hoc. g The representative Col II and MMP13 were detected by the immunofluorescence. Scale bar = 25 μm. Data shown as mean and error bar represents s.e.m.