Fig. 3

CD11b promotes miR-Let7a mediated immune stimulation. a Relative mRNA expression of pro-inflammatory and anti-inflammatory factors in WT (white bars) and Itgam−/− (blue bars) macrophages incubated with and without the Stat3 inhibitor 5,15 DPP; inset, Stat3 phosphorylation in WT and Itgam−/− macrophages (n = 2–3). b Relative mRNA expression of pro-inflammatory and anti-inflammatory factors in IL6-stimulated human (white bars) and murine (cyan bars) bone marrow-derived macrophages and murine total bone marrow derived myeloid cells (blue bars) (n = 3); p < 0.05 with these exceptions: mBMM (Ifng, Il12b); mCD11b+ (Arg1, Pdgfb, Il12b and Il1b); hBMM (Arg1, Ifng, Il1b). c Relative Il6 and Pdgfb mRNA expression in WT and Itgam−/− cells transduced with non-silencing (white bars or Il6 (blue bars) siRNA (n = 3). d Relative Let7a expression in murine macrophages transduced with non-silencing (white bars) or Itgam (blue bars) siRNAs, macrophages incubated with control IgG (white bars) or neutralizing anti-CD11b (blue bars) antibodies, and WT (white bars) or Itgam−/− (blue bars) macrophages (n = 3). e Time course of Let7a (left) and Il6 (right) expression in WT murine CD11b+ cells seeded on ICAM-1 (blue solid line) or maintained in suspension (black dotted line) (n = 3). f Relative expression of miRNA Let7a and Il6 in human macrophages adherent to ICAM-1 (blue) or maintained in suspension (white) (n = 3). g Relative mRNA expression of inflammatory factors in WT and Itgam-/- BMM transduced with control (white bars), pre-miRNA Let7a (cyan bars) or anti-miRNA Let7a (blue bars) (n = 3). h Relative Pdgfb and Vegfa expression in WT BMM transduced with control (white bars) or anti-miRNA Let7a (blue bars) (n = 3). i Time course of c-Myc expression in IL-4 or IFNγ + LPS stimulated WT (black lines) or Itgam−/− (blue lines) macrophages (n = 3). j Time course of c-Myc expression and pSer62myc phosphorylation in WT and Itgam−/− macrophages. k Relative mRNA expression of miRNAs Let7a (white bars), Let7d (blue bars) and Let7f (cyan bars) in basal, IL-4, or IL-4+ c-myc inhibitor treated WT and Itgam−/− macrophages (n = 3). l Relative mRNA expression of Il6, Arg1, and Pdgfb in IL-4 stimulated WT (white bars) and Itgam−/− (blue bars) macrophages treated with (blue bars) or without (white bars) c-Myc inhibitor 10058-F4. Error bars indicate sem. “n” indicates biological replicates. *p < 0.05 indicates statistical significance determined by Student’s t-test for a, d–f, and Anova with Tukey’s post-hoc testing for b, g, k. Source data are provided as a Source Data file