Fig. 4
From: Integrin CD11b activation drives anti-tumor innate immunity
Macrophage microRNA let-7a is required for tumor growth suppression. a, b Endothelial cells and vascular smooth muscle cells attached to microcarrier beads were cultured in fibrin gels containing WT or Itgam−/− BMMs transduced with control miRNA, pre-miRNA Let7a, anti-miRNA Let7a or Pdgf-bb siRNA. a Images b histograms of CD31+ positive vessel length (mm) (n = 10). c CD31 (green) and SMA (red) immunostaining of sections from in vivo cultured bFGF-saturated Matrigel plugs containing BMM transduced with control miR (black bars) or anti-miR Let7a (blue bars); quantification of the percentage of SMA+ vessels per matrigel plug (n = 25). d Schematic and graph of targeted delivery of anti-miR Let7a in animals with LLC tumors; tumor volumes from control anti-miRNA (black line) or anti-miRNA Let-7a (cyan line) treated animals (n = 10). e Let7a expression in cell populations sorted from peripheral blood cell and tumors from control (black bars) and anti-miRNA Let7-treated (cyan bars) animals from d (n = 3). f Relative mRNA expression of inflammatory factors in sorted macrophages from d (n = 3). g Representative images of CD31/Desmin co-localization and FITC-Dextran localization in treated tumors from d. h Quantification of the percentage of CD31/Desmin co-localization (n = 30) and of FITC-dextran leakage into tissues (n = 25). i CD4+ and CD8+ cells/field in tumors from control anti-miR (black bars) or anti-miR let-7a (blue bars) transduced animals (n = 25) scale bars, 40 µm. j Schematic representation of chemotherapeutic treatment in combination with targeted delivery of anti-miR-let7a. k Volumes and endpoint weights of LLC tumors in animals transduced with control anti-miR (black), anti-miR let-7a (blue), control anti-miR /Gemcitabine (green), and anti-miR let7a/Gemcitabine (red) (n = 10). Bar on micrographs indicates 50 µm. Error bars indicate sem. “n” indicates biological replicates. *p < 0.05 indicates statistical significance by Student’s t-test for c–i and by Anova with Tukey’s post-hoc testing for j Source data are provided as a Source Data file
