Fig. 6

An additional PDX model reveals clonal selection during metastasis. a In BC3_A2, unique dominant barcodes in each sample were quantified and plotted. The mean quantity of unique barcodes present in each site is depicted above each bar. (two-tailed T-test, *p = 9.40e−6; error bars are SEM; n = 4 MFP tumors, n = 7 lung metastases). b In BC3_A2, Shannon Diversity Indices were calculated (in nats: natural digits) taking into account all barcodes for each sample as a measure of ITH. (two-tailed T-test, *p = 7.55e−6; error bars are SEM; n = 4 MFP tumors, n = 7 lung metastases). c In BC3_A2, the percent of dominant barcodes (top 95%) in each metastatic lesion relative to the total number of unique barcodes detected in its matched MFP tumor is shown (labeled 3A–3D). d In BC3_A2, the percent of seeding clones detected in each metastatic lesion relative to the total number of unique barcodes detected in its matched MFP tumor is shown. e In BC3_A2, the number of dominant barcodes in metastases of each organ was compared with the total number of seeding clones detected in each metastasis. f In BC3_A2, the frequencies of the 10 most abundant barcodes detected across all samples within each mouse are plotted in different colors. Gray striped bars represent all other barcodes in each sample comprising the remainder of the population, and the numbers of these barcodes detected in each MFP tumor is written. Barcodes marked by the same colors in different mice are not identical to one another. Colored lines indicate expansion of primary tumor clones in metastatic lesions, although cross-metastasis seeding may have also occurred