Table 1 Limiting dilution transplantation to measure metastasis-initiating frequency

From: High-resolution clonal mapping of multi-organ metastasis in triple negative breast cancer

 

Cell number

Lung

Liver

Brain

PIM1-CBRluc

100

4/4 (100%)

1/3 (33%)

1/3 (33%)

PIM1-CBRluc

10

5/6 (83%)

2/3 (67%)

1/3 (33%)

PIM1-CBRluc

M.I.F.

17.9% (6.6–48.5%)

2.1% (0.6–7.6%)

0.7% (0.2–3.3%)

BC3_A2

100

3/5 (60%)

NT

NT

BC3_A2

10

0/8 (0%)

NT

NT

BC3_A2

M.I.F.

0.7% (0.2–2.4%)

N/A

N/A

  1. Of mice that grew MFP tumors upon transplantation of tumor cells in limiting dilutions, the frequency of metastasis was quantified with BLI ex vivo in two PDX models. The number of organs that harbored metastases is shown over the number of organs assayed by BLI, and the percent of positive organs is shown in parentheses. The estimated metastasis-initiating frequency (MIF, 95% confidence intervals shown in parentheses) is shown for each site
  2. NT: not tested, N/A: not applicable
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