Fig. 7
From: Quantifying post-transcriptional regulation in the development of Drosophila melanogaster
Hrb98DE post-transcriptionally regulates glucose metabolism. a Proteome changes upon in vivo knockdown of HRB98DE during Drosophila development. Bars show the percentage of all genes within each protein class that are differentially expressed upon Hrb98DE knockdown. Absolute numbers are also shown (differentially expressed proteins in class/number of all proteins in class). Significant overrepresentation of differential expression is observed in the rejected and delayed-production categories and preferentially contain an Hrb98DE motif in their mRNA sequence, when compared to the background of all expressed genes (left bar). * indicates p < 0.05 using a hypergeometric test. b Transcriptome and proteome changes upon knockdown of HRB98DE in S2R+ cells, shown as a scatterplot of mRNA vs. protein fold-changes. Significant cases of only mRNA changing (pink) or only protein changing (light blue) are highlighted. Dashed lines indicate thresholds of fold-changes for mRNA (absolute log2 fold-change > 1.3, BH corrected p value < 0.05) or protein (absolute log2 fold-change > 1.5, nominal p value < 0.01). c Heatmap of fold-changes for mRNA and protein level of differentially expressed genes upon Hrb98DE knockdown in S2R+ cells (in 14 cases mRNA and protein are both significantly changing, in 26 cases only significant change on protein level, 4149 with no change of mRNA or protein). For genes with no significant change in either mRNA or protein only a subset of 20 randomly chosen genes is shown. d Splicing changes at the mRNA level upon knockdown of HRB98DE in S2R+ cells. Venn diagram showing differentially spliced genes upon Hrb98DE knockdown and their overlap with genes previously identified as Hrb98DE targets or genes annotated as regulators of glucose metabolic processes (GO:0010906). The previously identified Hrb98DE targets were defined by combining Hrb98DE targets reported by Blanchette et al. (2009)41, Ji and Tulin (2016)40 and mRNAs with the Hrb98DE binding motif. e Differentially spliced genes are enriched for Hrb98DE targets (blue striped bar) or genes annotated as regulators of glucose metabolic processes (red striped bar), when compared to the background of all detected genes (clear bars). ** denotes significance with a p value below 0.01 (hypergeometric test)
