Fig. 5
Interactions between HaRxL103Emoy2/HaRxL103Hind2 and RPP4 in cytoplasm and/or nucleus. In planta interaction of GFP-HaRxL103Emoy2 (a), GFP-HaRxL103Hind2 (b), and GFP-NLS/nls-HaRxL103Hind2 (c, d) with RPP4-FLAG in cytoplasm and nucleus. Cytoplasmic (C) and nuclear (N) protein extracts were separately isolated from N. benthamiana leaves inoculated with Agrobacterium containing the indicated gene constructs at 2 dpi. Co-immunoprecipitation was performed with each extract using MACS MicroBeads with GFP antibody. Anti-GFP (upper panel), anti-FLAG (the 2nd panel), anti-UGPase (the 3rd panel), and anti-Histone H3 antibodies (bottom panel) were used to detect the related proteins in the immunoprecipitates. UGPase and Histone H3 were checked as markers for cytoplasmic and nuclear proteins, respectively
