Fig. 6
In an Emoy2/Cala2 F2, virulence consists with lack of HaRxL103 expression. a Expression of HaRxL103 during infection in Emoy2/Cala2 F2 individual progeny which showed virulence or avirulence on CW84:RPP4Col plants. Total RNA was prepared from the infections in Arabidopsis Ws-2 eds1-1 mutant at 2 or 4 dpi. qRT-PCR analysis was done as described in Fig. 1c. b Genotyping of the HaRxL103 locus in Emoy2/Cala2 F2 individual progeny which showed virulence on CW84:RPP4Col plants. Genotyping of the HaRxL103 locus was performed by a CAPS (cleaved amplified polymorphic sequence) method. Upper and bottom panels indicate PCR products and those after restriction enzyme (RE) treatment, respectively. M marker, ND not detectable
