Fig. 2
Specific HMOs are associated with symptomatic rotavirus infection. Orthogonal partial least squares-discriminatory analysis (OPLS-DA) within a secretors only and b non-secretors only show that the HMO profile of breast milk from mothers in the symptomatic rotavirus group are distinct from the asymptomatic and rotavirus negative groups. This distinction is mediated by differences in the levels of LNT, 2’FL, and LNFP I in secretors (c) and by LNT, 6’SL, and LNFP II in non-secretors (d). Bar graphs represent average levels of each HMO in nmol/ml with error bars indicating standard error of the mean. p value <0.05 (analysis of variance with Dunnett’s post hoc test) was considered statistically significant (asterisk (*)). The HMO profile of breast milk was predictive of symptomatic rotavirus infections as seen by receiver operating characteristic (ROC) curves generated by linear support vector machine (SVM) classification showing the area under the curve (AUC) with different numbers of HMOs included in the model within secretors (e) and non-secretors (f)
