Fig. 4
A natural antisense lncRNA regulates MAF4 gene expression. a Genome browser view of MAF4 and MAS. Signals from RNA- and sRNA-seq are shown, with normalized read counts per million along the y-axis. T-DNA insertion and amiRNA target sites are indicated. TSS, transcription start site; TTS, transcription termination site. b Detection of MAF4 and MAS in Col-0 after 0–28 d of cold exposure by RT-qPCR. c Detection of MAF4 and MAS in Col-0 and maf4-1 before (0 d) and after 20 d of cold exposure. d Detection of MAF4 and MAS in Col-0 and two MAF4 amiRNA knockdown lines (amiR-MAF4-1 and amiR-MAF4-2) before (0 d) and after 20 d of cold exposure. e Detection of MAS and MAF4 in Col-0 and two MAS amiRNA knockdown lines (amiR-MAS-1 and amiR-MAS-2) before (0 d) and after 20 d of cold exposure. In b–e, error bars represent s.e.m (n = 3), asterisks indicate a significant difference between the indicated groups (t-test, P-value < 0.05). f Flowering-time phenotypes of Col-0, maf4-1, and amiR-MAS-1/2 lines grown in SD conditions after 20 d of cold exposure. Numbers of primary rosette leaves were counted when bolts were ~ 3–5 cm long. At least thirty plants of each genotype were used for statistical analysis. Asterisks indicate a significant difference (t-test, P-value < 0.05). Source data are provided as a Source Data file
