Fig. 5
MAS regulates MAF4 at the transcriptional level. a Remaining levels of MAS, MAF4, and GAPDH at different time points post actinomycin D (100 μg/mL) treatment in Col-0 and amiR-MAS-1/2 lines. Plants were cold-treated for 20 d prior to actinomycin D treatment. b Transcription rates of MAS, MAF4, and ACTIN2 measured by nuclear run-on assay and RT-qPCR in Col-0 and amiR-MAS-1/2 lines after 20 d of cold exposure. c Upper panel, schematic representation of the construct used to generate the transgenic line expressing MAS under the control of a β-estrogen-inducible promoter. Lower panel, time-course of β-estradiol (20 μM)-inducible MAS and MAF4 expression as determined by RT-qPCR. d Transcription rates of MAS, MAF4, and ACTIN2 as measured by nuclear run-on assay in the transgenic line treated with β-estradiol for 24 h. e Detection of MAS and MAF4 in Col-0 and two transgenic lines overexpressing MAS (MAS OX-1 and MAS OX-2). In b–e, error bars represent s.e.m (n = 3-4), asterisks indicate a significant difference (t-test, P-value < 0.05). Source data are provided as a Source Data file
