Table 1 Data collection and structure refinement statistics
From: A quaternary tetramer assembly inhibits the deubiquitinating activity of USP25
Data collection | ||
Beamline | ALBA-XALOC | ALBA-XALOC (Hg2+) |
Space group | I422 | I422 |
Wavelength (Å) | 0.9791 | 0.9998 |
Resolution (Å) | 95.08–3.28 (3.29–3.28)a | 94.76–4.35 (4.35–4.37) |
a, b, c (Å) | 140.806, 140.806, 190.156 | 141.17,141.17,190,17 |
α, β, γ (°) | 90, 90, 90 | 90, 90, 90 |
Unique reflections | 14,883 | 6588 |
Data redundancy | 6.50 (6.40) | 25.4 (26.5) |
Rmerge | 0.07 (0.98) | 0.19 (1.06) |
CC (1/2) | 0.99 (0.85) | 0.99 (0.97) |
I/σ | 16.3 (2.3) | 20.3 (6.9) |
Completeness (%) | 99.5 (97.7) | 99.9 (95.7) |
Refinement | ||
Resolution (Å) | 95.08–3.28 (3.37–3.28) | |
Non-anomalous reflections | 14,150 | |
Rwork/Rfree | 0.202/0.275 | |
Number of all atoms | 3963 | |
B-factor protein (Å2) | 129.6 | |
RMSD bond (Å)/angle (°) | 0.011/1.524 | |
Ramachandran plot | ||
Favored (%) | 91.99 | |
Allowed (%) | 6.71 | |
Disallowed (%) | 1.30 |
