Fig. 6

Silencing of Nsd2 abrogates metastasis in vivo. Panels a–f show in vivo analyses of Nsd2 silencing in a mouse metastatic cell line (NPK cells). Cells (1 × 10⁶ cells) were engrafted subcutaneously into the flank of nude mice and the mice were monitored for up to 40 days. Studies were done using 2 independent shRNA for Nsd2; representative data for shRNA#1 is shown. a Survival analyses with the endpoint being tumor volume of 1.5 cm³ (n = 10/group). The p-value was calculated using a log-rank test. b Analyses of tumor weights (in grams) at the time of killing (total n = 9/group). c Number of lung metastases per mouse (total n = 9/group). b, c p-values were calculated using the Mann–Whitney U test; error bars represent the standard deviation (s.d.) from the mean. d Representative whole mount and epifluorescence images of lung metastases. Scale bar represent 100 microns. e Representative immunostaining of shControl and shNsd2 tumors using the indicated antibodies (n = 4/group). Scale bars represent 50 μ. f Western blot analysis showing representative cases from the shControl (lanes 1, 2) and shNsd2 (lanes 3, 4) tumors using the indicated antibodies (total n = 4/group). The position of a molecular marker is shown; uncropped images are provided in Supplementary Figure 5