Fig. 7

uPARAP downregulation induces Rac1 over-activation upon VEGF-C stimulation. a, b Cdc42 (a) and Rac1 (b) time course activation in uPARAP-silenced (siU) or control (Ctr) LECs. For pull-down quantification, values were calculated as the ratio of Rac1-GTP to total Rac1 form and normalised to the values obtained with the control (Ctr) (n = 4, biological replicates). c Gelatin sponges soaked with VEGF-C were implanted in mouse ears. Sponges were injected or not with Rac1 inhibitor, NSC 23766 (NSC). Lymphatic vasculature was examined by LYVE-1 (green) immunostaining. Bars = 500 µm. Histogram represents the area density of vessels quantified by a computer-assisted method and expressed as percentage of WT control. Values correspond to the mean (n = 10) ± SEM. Statistical analyses were performed using a non-parametric Mann–Whitney test. *P < 0.05, **P < 0.01, and ***P < 0.001