Fig. 2
From: Ribosome-induced RNA conformational changes in a viral 3′-UTR sense and regulate translation levels
TYMV 3′-UTR context alters the rate of translation. a Luciferase (LUC) reporter design. Elements that were altered are indicated. The top reporter represents the 0 nt spacer, the middle reporter represents different spacer nucleotide lengths, and the bottom reporter represents the LUC stop codon mutant reporter (indicated by the magenta symbol). The red symbol represents the LUC stop codon and the stop codon that is inside the UPD. Reporters were capped unless indicated. b–d In vitro translation assays with: b two different spacers between the two stop codons; c two spacer lengths and the upstream stop codon mutated; d four spacer lengths with data showing the mean translation levels for n ≥ 3 replicates. Absolute differences in LUC activity between panels are due to different amounts of lysate used. Error bars represent one s.e.m. of n ≥ 3 replicates. Sequence details in Supplementary Fig. 2
