Fig. 4
From: Ribosome-induced RNA conformational changes in a viral 3′-UTR sense and regulate translation levels
Functional coupling between RNA domains. a Secondary structures of the U8A and G10C mutants, colored to reflect the difference in chemical probing between mutant and WT. The new secondary structure is derived from the x-ray crystal structure (see refs. 9,36 for previously predicted secondary structures). Base shading represents NMIA probing and tick marks represent DMS or CMCT probing, colored as in Fig. 3b. b Left: in vitro valylation assay comparing mutants U8A, U24A, the double mutant U8A + U24A, G10C, and the compensatory mutant G10C + C22G to WT and a mutant with altered anticodon loop (ACmut) to inhibit valylation. Right: Translation assay comparing the U8A and G10C mutants to WT RNA reporters used to mimic the gRNA (blue) and sgRNA (red). Error bars represent one s.e.m. of n≥ 3 replicates
