Fig. 7
From: Ribosome-induced RNA conformational changes in a viral 3′-UTR sense and regulate translation levels
Interdomain communication and the spine structure. a Stereoview of the linker region and surrounding structure. Yellow spheres indicate the O4′ atom to highlight the double inversion. Grey hashes: stacking. The Z-DNA-like motif is found in the syn-G27 and A26 step. b In vitro translation assays of a reporter with the interdomain linker length increased to 12 nucleotides. Data from two different spacers is included for comparison. Error bars represent one s.e.m. of n ≥ 3 replicates. c Left: A spine of continuously stacked bases extends from the UPD to the T-loop. Nucleotides in the spine are shown in stick representation (colored to match Fig. 5, G10C and U8A positions are shown). Right: Secondary structure of the TLS + UPD, with nucleotides visible to the left highlighted and colored to match. The stop codon in the UPD is boxed in black. The location of the leading edge of the ribosome when the stop codon is in the A site is shown. d Surface representation of the structure colored by relative crystallographic B-factor, with red highest and blue lowest
