Fig. 1
From: Control of Treg cell homeostasis and immune equilibrium by Lkb1 in dendritic cells
DC-specific deletion of Lkb1 leads to specific enlargement of Treg cell pool. a Flow cytometric analysis of Treg cell frequencies among splenic CD4+ T cells from Lkb1f/f and Cd11cCreLkb1f/f mice. b Quantification of the percentage and absolute number of Treg cells in the spleen and lymph nodes (LNs) from Lkb1f/f (red dots) and Cd11cCreLkb1f/f (blue dots) mice of different ages. c, d Flow cytometric analysis (c) and quantification (d) of Ki67 expression in splenic Treg cells from Lkb1f/f and Cd11cCreLkb1f/f mice. e Flow cytometric analysis of Treg cell frequencies among CD4+ T cells in the blood, bone marrow (BM), lungs, liver, kidneys and brain of Lkb1f/f and Cd11cCreLkb1f/f mice. f Quantification of the percentages of Treg cells among CD4+ T cells in the blood, BM, lungs, liver, kidneys and brain of Lkb1f/f (red dots) and Cd11cCreLkb1f/f (blue dots) mice. g Flow cytometric analysis of CD25, CD44, CD62L, Helios, CD73, ICOS, Nrp1 and CTLA4 expression on splenic Treg cells from Lkb1f/f (red line) and Cd11cCreLkb1f/f (blue line) mice. h Suppression assay in which CFSE-labelled naïve T (Tn) cells were co-cultured with Treg cells from Lkb1f/f and Cd11cCreLkb1f/f mice at a 4:1 ratio. Each symbol (b, f) indicates an individual mouse; the results are presented as the mean ± S.E.M., *P < 0.05, **P < 0.01, ***P < 0.001, by Student's t-test (b, d, f). Data are pooled from b, d, f or are representative of a, c, e, g, h three independent experiments with similar results
