Fig. 2
From: Control of Treg cell homeostasis and immune equilibrium by Lkb1 in dendritic cells
Impaired adaptive immune responses due to increased Treg cell number. a Disease-related clinical scores of Lkb1f/f and Cd11cCreLkb1f/f mice subjected to MOG35–55-induced experimental autoimmune encephalomyelitis (EAE). b, c Flow cytometric analysis (b) and quantification (c) of IFN-γ- and IL-17-producing CD4+Foxp3− T cells in the spleen and brain of Lkb1f/f and Cd11cCreLkb1f/f mice with EAE. d–f Flow cytometric analysis (d), percentage (e) and absolute number (f) of CFSE-labelled CD4+ T cells (2×106) from OT-II mice transferred into Lkb1f/f, Cd11cCreLkb1f/f, Lkb1f/fFoxp3DTR and Cd11cCreLkb1f/fFoxp3DTR mice treated with diphtheria toxin (DT) (50μgkg−1, 2 days in advance). g, h Flow cytometric analysis (g) and quantification (h) of IFN-γ-producing OT-II T cells in Lkb1f/f, Cd11cCreLkb1f/f, Lkb1f/fFoxp3DTR and Cd11cCreLkb1f/fFoxp3DTR mice stimulated with phorbol myristate acetate (PMA) and ionomycin for 4 h. The results are presented as the mean ± S.E.M., *P < 0.05, **P < 0.01, by two-way ANOVA (a) or Student's t-test (c, e, f, h). Data are pooled from c, e, f, h or are representative of a, b, d, g three independent experiments with similar results
