Fig. 4
From: Control of Treg cell homeostasis and immune equilibrium by Lkb1 in dendritic cells
Lkb1-deficient DCs promote Treg cell expansion through the OX40L-OX40 axis. a Gene set enrichment analysis (GSEA) of transcriptional profiles in WT and Lkb1-deficient DCs. The gene set of cell-cell adhesion was enriched in Cd11cCreLkb1f/f DCs. FDR, false-discovery rate; NES, normalized enrichment score. b Volcano plot analysis of gene expression in splenic DCs from Lkb1f/f and Cd11cCreLkb1f/f mice. The points indicate the Log2 fold change (x axis) versus the –Log10 P value (y axis, representing the probability that the gene is differentially expressed). Black dots mark genes with P > 0.1 and less than two-fold change, and tinted dots mark genes with fold changes higher than 2. Ox40l is marked. c mRNA level of Ox40l in Lkb1f/f and Cd11cCreLkb1f/f DCs determined by real-time PCR. d Flow cytometric analysis and quantification of the relative mean fluorescence intensity (MFI) of OX40L expression on DCs from the spleen and LNs of Lkb1f/f and Cd11cCreLkb1f/f mice. e–g Flow cytometric analysis (e), percentage (f), and absolute number (g) of CFSE-labelled Treg cells (CD4+Foxp3+, 2×105) sorted from B6 mice after 4 days of co-culture with DCs (1×105) sorted from Lkb1f/f and Cd11cCreLkb1f/f mice pre-blocked with OX40L neutralizing antibodies or isotype control. h–j Flow cytometric analysis (h), percentage (i) and number (j) of CFSE-labelled Treg cells (CD4+Foxp3+, 2×106) sorted from B6 mice transferred together with or without DCs (1×106) sorted from Lkb1f/f or Cd11cCreLkb1f/f mice pre-blocked with OX40L neutralizing antibodies or isotype control into irradiated NSG mice via tail vein injection for 3 days. The results are presented as the mean ± S.E.M., *P < 0.05, **P < 0.01, ***P < 0.001, by Student's t-test (c, d, f, g, i, j). Data are pooled from c, d, f, g, i, j or are representative of at least three e, h independent experiments with similar results
