Fig. 6
From: Control of Treg cell homeostasis and immune equilibrium by Lkb1 in dendritic cells
LPS induces Treg cell expansion and suppression through depleting Lkb1 in DCs. a Lkb1 protein in splenic DCs from Lkb1f/f (WT) and Cd11cCreLkb1f/f (CKO) mice treated or untreated with LPS (1.5 mg kg−1) overnight. b mRNA level of Lkb1 in splenic DCs from mice with or without LPS treatment. c Representative Treg cell frequencies among splenic CD4+ T cells from mice treated with or without LPS 5 days in advance. d Quantification of the percentage and absolute number of Treg cells in the spleen and LNs of mice treated with or without LPS 5 days in advance (Ctrl, n=8; LPS, n=10). e Expression of Nrp1 and Helios in splenic Treg cells from mice with or without LPS treatment 5 days in advance. f The survival curve of Lkb1f/f (n=8 each group) and Lkb1f/fFoxp3DTR mice (n=6 each group) pre-treated with or without LPS (5 days in advance), treated with DT (50 μg kg−1, 2 days in advance), and then challenged with a lethal dose of LPS (30 mg kg−1). g–i Flow cytometric analysis (g), percentage (h) and number (i) of CFSE-labelled Treg cells sorted from B6 mice after 4 days of co-culture with DCs sorted from C57 mice with or without LPS (1.5 mg kg−1) treatment pre-blocked with OX40L neutralizing antibodies or isotype control. j The survival curve of Lkb1f/f (n = 12), Cd11cCreLkb1f/f (n = 13), and Cd11cCreLkb1f/fFoxp3DTR (n = 14) mice treated with DT and then challenged with a lethal dose of LPS. Each symbol (d) indicates an individual mouse; the results are presented as the mean ± S.E.M., **P < 0.01, ***P < 0.001; NS not significant, by Student's t-test (b, d, h, i). Log-rank survival curve analysis was used (f, j). Data are pooled from b, d or are representative of a, c, e, g three independent experiments
