Fig. 2

Plasma redox imbalance induces NETosis. a Concentration of free thiol in plasma from wild-type (WT) vs. albumin deficient (Alb^−/−) C57BL/6 mice and control vs. albumin-depleted plasma from NOD scid gamma (NSG) and BALB/c mice. b–d Plasma isolated from the blood of WT and Alb^−/− C57BL/6 mice and NSG mice 2 days post saline, IAA (30 mg/kg), or LPS (8 mg/kg) injection was subjected to FPLC (b, c) and Western blotting (d). b Representative FPLC results for the redox state of albumin in C57BL/6 (left) and NSG (right) mice. c The ratio of peak intensity for reduced/oxidized albumin in NSG mice. d Western blotting with an antibody against C-reactive protein (CRP) and serum amyloid P component (SAP). e–g Plasma was isolated from the blood of WT and Alb^−/− C57BL/6 mice and NSG mice 2 days post saline or IAA (30  mg/kg) injection following intravenous injection of PBS or murine albumin (20 mg/mouse). e, f Immunoprecipitation of cell-free DNA in the plasma from C57BL/6 (e) and NSG (f) mice using an anti-dsDNA antibody followed by Western blotting using an antibody against citrullinated histone H3 (Cit-H3). g Plasma free thiol concentration. a, c, g Results represent individual values with the mean ± s.d. (n = 3–6; biological replicates, *P < 0.05 by Dunnett’s test)