Fig. 5
From: Hemocyanin facilitates lignocellulose digestion by wood-boring marine crustaceans
Digestibility of willow following short hemocyanin pretreatments at room temperature. Digestibility of willow measured as release of cellobiose (nmoles) per unit biomass (mg) in saccharification reactions upon biomass pretreatment. Pretreatment of willow with hemocyanin in seawater (Hc, dark tones) or seawater alone (SW, light tones) without (blue colors) and with ( + , red colors) 50 mm DTPA (chelator) for 10 min (a) or 50 mm dithionite (reducing agent) for 20 min (b), followed by saccharification with CBH I from Hypocrea jecorina (HjCBH I). Hydrolysates were analyzed by HPAEC against cello-oligomer standards, and values normalized to reactions without CBH following pretreatments to account for any remaining cellulase activity derived from the hemocyanin preparation. Circles represent sample values and bars sample mean ± SD, N = 3 (N = 2 for Hc ± DTPA)
