Fig. 4
From: A scalable peptide-GPCR language for engineering multicellular communication
The synthetic communication language enables construction of an interdependent microbial community. a Illustration of the interdependent microbial communities mediated by the peptide-based synthetic communication language. Peptide-signal interdependence was achieved by placing an essential gene (SEC4) under GPCR control. In the featured three-yeast ring c1, c2, and c3 secret the peptide needed for growth of the cx-1 member of the ring. Peptides are secreted from the constitutive ADH1 promoter. b, c Growth of the three-membered interdependent microbial community over > 7 days. Communities with one essential member dropped out collapse after ~ 2 days (c). Three-membered communities were seeded in a 1:1:1 ratio, controls were seeded using the same cell numbers for each member as for the three-membered community. All experiments were run in triplicate and error bars represent the standard deviation. d The composition of the culture was tracked over time by taking samples from one of the triplicates at the indicated time points, plating the cells on media selective for each of the three component strains, and colony counting
