Fig. 6
Zelda hubs kinetic properties. a Living GFP-zld embryo imaged by confocal microscopy from interphase of nc11 to early interphase of nc14. Successive representative maximum intensity projected Z-stack images showing local inhomogeneities are shown at each cycle (see also Supplementary Movie 11). Scale bars represent 10 μm. b Nuclei zoom from living GFP-zld embryos imaged by confocal microscopy from interphase of nc11 to early interphase of nc13. Successive representative images showing local hubs are shown at each cycle. Scale bars represent 1 μm. c Nuclei zoom from living GFP-zld/mCherry-Zld embryos imaged by confocal microscopy at interphase of early nc11. Successive representative images showing Zelda subnuclear hubs are shown at each cycle (an example is indicated by an arrow). Scale bars represent 5μm. d Living GFP-Zld/ + ;MCP-RFPt/ + embryo containing the snaE + 1Zld3’ transgene (MS2 in magenta) imaged by confocal microscopy. Successive representative maximum intensity projected Z-stacks of slices containing the MS2 signal only (see also Supplementary Movie 13). Scale bars represent 5 μm. e Example of a FRAP experiment on a single hub. f FRAP mean curve (black) and the mean of all the fits (red curve) using diffusion reaction models determined at the bleached spot for 11 nuclei from nc10–13 developing GFP-zld embryos. Error bars represent SD from different nuclei (light blue bars). Scale bars represent 5 μm. g Box plot representing estimated residence times (1/koff) extracted with diffusion reaction model of GFP-Zld in Global (dark green) compared to Hubs (light green). Numbers above each plot represent the mean RT. Centered lines represent the median and whiskers represent min and max
