Fig. 1

Curation of metagenomic data. a Schematic representation of the workflow for removal of artefacts introduced during genomic extraction or preparation of sequence libraries in the low-biomass neonatal samples. b Sample-wise bioinformatic workflow for removal of artefactual sequences from metagenomic data, extraction of taxonomic and functional profiles, and reconstruction of genomes and strain-resolved analyses. The resulting data sets used for inter-sample comparisons are highlighted in grey. mOTU, metagenomic operational taxonomic unit