Fig. 5
Topical application of IS-2-Pi-TPP ointment to treat C. albicans murine skin infections. a Abraded murine skin was inoculated with 107 C. albicans SC5314 cells. After 24 h, the skin was treated with ointment containing no drug (vehicle) or 0.25%, 0.5%, 1%, or 2% (w/w) IS-2-Pi-TPP. Ointments containing 1% FLC or nystatin were used as positive controls. The fungal burden in the skin is expressed as the number of viable C. albicans cells (log10 CFUs per gram) in 4–7 skin samples for each group of mice (seven mice in the vehicle control, four mice in the treated groups). Each circle represents one skin sample. The two-tailed unpaired t-test was performed. *P < 0.05, ***P < 0.001 indicate significance. b Micrographs of PAS-stained and H&E-stained skin biopsies after the indicated treatments. Scale bar, 100 μm. c Evaluation of the skin toxicity of IS-2-Pi-TPP. Ointment containing 8% IS-2-Pi-TPP was applied on abraded murine skin. H&E staining was used to detect lymphohistiocytic infiltrates under IS-2-Pi-TPP treatment. Scale bar, 100 μm
