Fig. 3

Absence of satellite cell differentiation in regenerating TA muscles of Islr cKO mice. a Immunofluorescence analysis of eMyHC⁺ fibers in TA muscles of control and Islr cKO mice at 3.5 (N = 3) and 5 (N = 3) d postinjury. The percentage of area occupied by eMyHC⁺ fibers per field at 5 d postinjury is shown on the right. b Immunofluorescence analysis of MyoG⁺ cells in TA muscles of control and Islr cKO mice at 5 d post injury. N = 3 in each group. The number of MyoG⁺ cells per myofiber is shown on the right. c Western blot analysis of eMyHC and MyoG protein levels in control and Islr cKO mice at 3.5 d postinjury. d Western blot analysis of eMyHC and MyoG protein levels in control and Islr cKO mice at 5 d postinjury. Scale bars are all 50 μm. Error bars represent the means ± s.d. ^**P < 0.01, ^***P < 0.001; Student’s t test