Fig. 3

FSD1 forms a ring structure at the subdistal region of both centrioles. a Schematic of centrosome structure. The centrosome is composed of a pair of centrioles, and each centriole is divided into three portions, including proximal end (red), middle region (green), and distal end (blue). The older centriole of the pair (the mother) is often decorated with distal appendages and subdistal appendages. During ciliogenesis, the transition zone extends from the distal end of the mother centriole. b FSD1 was visualized with centriolar proximal (C-Nap1), distal (Cep162, Centrin2, and IFT88), subdistal appendage (ODF2), and distal appendage (Cep164) markers. ODF2, IFT88, and Cep164 localize to mother centrioles. Scale bar, 500 nm. c The centriolar localization of FSD1 was observed in ciliated and non-ciliated cells stained with indicated antibodies. Cep162, the centriole distal end marker. ARL13B, the primary cilia marker. Scale bars, 5 μm (main image) and 500 nm (magnified region). d The centriolar localization of FSD1 was observed in ciliated cells stained with indicated antibodies. Cep162, the centriole distal end marker. MKS1 and NPHP8, the ciliary transition zone markers. Scale bar, 500 nm. e RPE-1 cells were transfected with mCherry-Centrin2 plasmids and stained with the indicated antibodies. The samples were then imaged using the stimulated emission depletion microscopy (STED). Cep164 and FBF1 localize to the distal appendage of mother centrioles. Scale bars, 500 nm. f Schematic representation of the centrosome illustrates that FSD1 forms a ring structure at the middle region encircling both centrioles