Fig. 4

The maintenance of Cep290 by FSD1 at CS is required for TZ assembly. a Non-ciliated (+Serum) or ciliated (−Serum) RPE-1 cells were stained with indicated antibodies as shown. Scale bars, 5 μm (main image) and 1 μm (magnified region). b Effects of FSD1 depletion on the localization of Cep290 at centriolar satellites in cycling cells. RPE-1 cells transfected with control or FSD1 siRNAs were stained with anti-Cep290 (green) and anti-Centrin2 (red) antibodies. Scale bars, 5 μm (main image) and 1 μm (magnified region). c Effects of FSD1 depletion on the PCM1 localization at centriolar satellites in cycling RPE-1 cells. RPE-1 cells transfected with control or FSD1 siRNAs were stained with indicated antibodies. Scale bars, 5 μm (main image) and 1 μm (magnified region). d Effects of FSD1 or PCM1 depletion on the Cep290 localization at centriolar satellites in cycling RPE-1 cells. e, f Effects of FSD1 or PCM1 depletion on the relative intensity of the transition zone components TMEM67 (e) and NPHP8 (f) at mother centrioles in quiescent cells. g Model accounting for the TZ assembly defects caused by FSD1 depletion due to the disruption of CS Cep290. TZ transition zone, CS centriolar satellite. Data are presented as mean ± s.d. of three independent experiments. n number of cells. In all panels, statistical comparisons between two groups were carried out by two-tailed t-test. **P < 0.01, ***P < 0.001