Fig. 7
From: Microtubule asters anchored by FSD1 control axoneme assembly and ciliogenesis
CAP350-FOP complex are essential for FSD1 centrosome localization. a Effects of depletion of known 12 anchoring proteins on the FSD1 localization at centrosomes. RPE-1 cells were transfected with indicated siRNAs and stained with antibodies to FSD1 (green) and γ-tubulin (red). Bars indicate the median. Significance between two groups was determined by two-tailed t-test. ***P < 0.001. Scale bar, 500 nm. b Immunofluorescence analysis of the relationship between FSD1 and the CAP350-FOP complex. RPE-1 cells transfected with the indicated siRNAs were stained with antibodies to CEP19, CAP350, or FOP (green), and to Centrin2 (red). Scale bar, 500 nm. c Immunoprecipitation and immunoblot analysis of the interaction of Flag-FSD1 with endogenous FOP, CAP350, and CEP19 in HEK293T cells. HEK293T cells were transfected with Flag-tagged FSD1; lysates immunoprecipitated with anti-Flag, as well as input lysates, were analyzed by immunoblot with indicated antibodies. d The interaction between endogenous FSD1 and CAP350, FOP in RPE-1 cells. Immunoblot analysis of lysates of RPE-1 cells immunoprecipitated with anti-FSD1, anti-CAP350, anti-FOP, or control IgG. e Model depicting the interactions between FSD1 and the CAP350-FOP complex. The size of arrowhead represents the affected strength on corresponding centrosome localization
