Fig. 5

Depletion of spleen CD11b^lo F4/80^hi macrophages in adult FLT3-deficient mice results in loss of DCs. a Schematic outline of the experiment. b Clodronate liposomes (Clod) were injected into Flt3^−/− and wild-type (WT) mice and the efficient depletion of blood monocytes (CD11b⁺ Gr-1^lo) was analyzed 24 h later (left). c Plot shows the reduction of blood monocytes. Data are pooled from two independent experiments. SD is shown. d Cell numbers of spleen macrophages (left), RP-Mps (middle), and DCs (right) normalized to non-injected controls from the same experiments 1–2 weeks after Clod injection. All cell types were depleted efficiently. Data are pooled from two independent experiments. SD is shown. e Cell numbers of spleen macrophages (left), RP-Mps (middle), and DCs (right) normalized to non-injected controls from the same experiments 4 weeks after Clod injection. Macrophages recovered in all mice. RP-Mps failed to recover in all mice. DCs recovered in Flt3-proficient but not in Flt3-deficient mice. Two-sided t tests were performed and SD is shown throughout the figure. SD is shown