Fig. 5
From: Binding of HMGN proteins to cell specific enhancers stabilizes cell identity
HMGN depleted iPSCs maintain pluripotency and differentiation potential. a Characterization of iPSCs colonies generated from WT and DKO MEFs. ALP: alkaline phosphatase assay; four bottom rows: immunofluorescence analyses of the indicated targets. DAPI visualizes DNA. Scale bar: 100 μm. b Scatter plot showing correlation between gene expression levels of WT and DKO iPSCs (average of 3 biological replicates). c Expression of select pluripotency markers in WT and DKO ESCs and in iPSCs (data was obtained from 3 biological replicates, and bar graph represents mean ± SD). d Teratomas derived from WT and DKO iPSCs injected into opposite flanks of nude mice. Scale bar: 1 cm. e Equal growth rate of teratomas derived from WT and DKO iPSCs (data was obtained from 18 replicates; error bars are mean ± SD). f Histological analyses of teratoma sections derived by injecting WT and DKO iPSCs into opposite flanks of nude mice. The three germ layers are identified by morphology after H&E staining (left) and by immunofluorescence with the indicated antibodies (right). Scale bar: 200 μm. g Scatter plot showing correlation between gene expression in teratomas derived from WT and DKO iPSCs (average of 6 biological replicates for each genotype)
